https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45113 Wed 26 Oct 2022 13:33:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45081 −/−) have increased baseline anxiety behaviors, SERT^+/− rats with low SERT expression (and more relevant to the clinical condition with low SERT expression) do not. Yet, no systematic studies of fear acquisition/extinction or their underlying neural mechanisms have been conducted in this preclinical genetic SERT^+/− model. Here we sought to determine if SERT^+/− or SERT^−/−, compared to wildtype, rats would show exacerbated panic responses and/or persistent conditioned fear responses that may be associated with PTSD or phobia vulnerability. Results: Only SERT^−/− rats showed increased baseline anxiety-like behaviors with heightened panic respiratory responses. However SERT^+/− (also SERT^-/-) rats showed enhanced acquisition of fear and delayed extinction of fear that was associated with changes in serotonergic-related genes (e.g., reduced 5-HT1A receptor) and disrupted inhibition within the basolateral amygdala (BLA). Furthermore, the disrupted fear responses in SERT^+/− rats were normalized with 5HT1A antagonist infusions into the BLA. Enhanced acquisition and failure to extinguish fear memories displayed by both SERT^−/− and SERT^+/− rats are cardinal symptoms of disabling anxiety disorders such as phobias and PTSD. The data here support the hypothesis that reduced SERT function is a genetic risk that disrupts select gene expression and network properties in the amygdala that could result in vulnerability to these syndromes.]]> Wed 26 Oct 2022 12:02:46 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:55901 Wed 03 Jul 2024 15:54:34 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43656  17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.]]> Tue 27 Sep 2022 14:32:55 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36575 Tue 09 Jun 2020 11:47:41 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36576 Tue 09 Jun 2020 11:40:47 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38058 + ions maintain a complete hydration shell while passing between the transmembrane cavity and cytosol, which must be accommodated. To put the canonical model to the test, we locked the conformation of a Kir K⁺ channel to prevent widening of the narrow collar. Unexpectedly, conduction was unimpaired in the locked channels. In parallel, we employed all-atom molecular dynamics to simulate K⁺ ions moving along the conduction pathway between the lower cavity and cytosol. During simulations, the constriction did not significantly widen. Instead, transient loss of some water molecules facilitated K⁺ permeation through the collar. The low free energy barrier to partial dehydration in the absence of conformational change indicates Kir channels are not gated by the canonical mechanism.]]> Thu 29 Jul 2021 12:11:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1017 Sat 24 Mar 2018 08:29:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:12665 diff< 0001). The circulatory changes thus appear to conserve body oxygen stores during the induced apnoea, an effect dependent on the integration of evoked vagal and sympathetic autonomic activity.]]> Sat 24 Mar 2018 08:15:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40151 5 mm) plastic residues. We have found that microplastics can be generated by simple tasks in our daily lives such as by scissoring with scissors, tearing with hands, cutting with knives or twisting manually, to open plastics containers/bags/tapes/caps. These processes can generate about 0.46–250 microplastic/cm. This amount is dependent on the conditions such as stiffness, thickness, anisotropy, the density of plastic materials and the size of microplastics. This finding sends an important warning, that we must be careful when opening plastic packaging, if we are concerned about microplastics and care about reducing microplastics contamination.]]> Fri 22 Jul 2022 13:55:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39651 Fri 17 Jun 2022 13:19:10 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39973 2) at baseline. Subjects/methods: A total of 4083 young women (27–31 years) in the healthy weight range (≥18.5 BMI <25 kg/m²) enroled in the Australian Longitudinal study on Women’s Health (ALSWH) were analysed. Diet quality was measured by the Australian Recommended Food Score (ARFS) and the Fruit and Vegetable Index (FAVI) using dietary data derived from a validated food frequency questionnaire. Weight change was calculated as the difference between baseline and 6-year follow-up weight (kg). Multiple linear regression models were used to analyse the association between baseline ARFS and FAVI and 6-year weight change. Results: At baseline, mean diet quality was low for both indices [ARFS (maximum 72) = 29.9 and FAVI (maximum 333) = 94.2] and women gained 3.7 kg of weight during 6 years of follow-up. Regression modelling revealed that every one point increase over 6 years in either the ARFS or FAVI score was associated with statistically significantly less weight gain over 6 years, although the amount was small (33 and 12 g, respectively). Conclusions: Higher diet quality predicts lower prospective weight gain in young women however, further research is needed over a longer follow-up period and in diverse population groups.]]> Fri 15 Jul 2022 10:18:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39981 Helicobacter pylori microbiota might account for some cases with unexplained chronic gastritis that may in a minority eventually progress to gastric cancer through the Correa cascade. We characterized gastric microbiota by describing the normal stomach, compared it with early precancerous lesions and other disease states, and assessed whether H. pylori status affects bacterial diversity. Methods: In a population-based study of those with and without gastrointestinal symptoms, cytology brush samples were collected during endoscopy from 316 individuals. Mucosal status was classified as normal mucosa (171), nonatrophic H. pylori gastritis (33), atrophic gastritis (12), or antral chemical gastritis (61). The 16S rRNA gene sequencing and analysis were performed to characterize the microbiota. Results: Microbiota in atrophic gastritis and nonatrophic H. pylori gastritis stomachs were dysbiotic and differed from those in the normal stomach (P = 0.001). The normal stomach had the highest microbial diversity, followed by antral chemical gastritis. The atrophic gastritis and chronic H. pylori gastritis groups had the lowest diversity, a difference that was statistically significant (P = 0.01). Besides H. pylori, non–H. pylori bacteria accounted for group differences. Microbial network analysis showed that the normal group network was most highly connected, whereas the H. pylori gastritis group had the lowest connection. We found an increasing positive co-occurrence of oral bacteria in the stomach because samples deviated from the normal network, some of which were pathogens. The H. pylori–negative group had the highest microbial diversity (Shannon index) compared with the H. pylori–positive group (P = 0.001). Discussion: In this low–H. pylori prevalence general population, the gastric mucosal microbiota of the normal stomach differed significantly from those with nonatrophic or atrophic gastritis. There was an increasing abundance of pathogenic bacteria from the normal state to early precancerous states.]]> Fri 15 Jul 2022 10:16:11 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39992 p < 0.001). Many survivors and parents have unmet needs for genetics-related services and information. Greater access to services and information might allow survivors at high risk for late effects to detect and prevent side effects early and improve medical outcomes. Addressing families’ needs and preferences during survivorship may increase satisfaction with survivorship care.]]> Fri 15 Jul 2022 10:13:46 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34107 T CC genotype has been previously linked to FD and TT genotype to antidepressant response in depression. The ss genotype of the 5-HTT LPR variant of the serotonin transporter gene (SLC6A4) has been linked to selective serotonin reuptake inhibitor (SSRI) response. We aimed to examine whether GNβ3 825C>T and 5-HTT LPR polymorphisms result in differential treatment effects in FD patients receiving antidepressant therapy. Methods: Participants were randomized to receive placebo, 50 mg AMI, or 10 mg escitalopram (ESC). The primary end point was adequate relief for ≥5 weeks of the last 10 weeks. Genotyping of GNβ3 825C>T and 5-HTT LPR was performed utilizing PCR-based methods. Results: GNβ3 825C>T and 5-HTT LPR genotype data were available for 256 (88%) and 246 (84%) patients, respectively. Both polymorphisms were in Hardy-Weinberg equilibrium. In tests for differential treatment, neither 5-HTT LPR nor GNβ3 825C>T genotype influenced response to therapy (P=0.89 and P=0.54, respectively). Although there was a tendency for a more favorable response to ESC in the SS/LS genotype compared to the LL genotype groups (40% vs. 31% reporting adequate relief of FD symptoms) among those in the ESC treatment arm, this was not significant (P=0.43). Conclusions: GNβ3 825C>T and 5-HTT LPR genetic variants do not alter treatment response to tricyclic and SSRI antidepressants in FD.]]> Fri 08 Feb 2019 10:43:58 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32705 Fri 03 Dec 2021 10:33:11 AEDT ]]>